LABORATORY TEACHINGS 2

Department of Medicine, CHS

The document outlines visual and colorimetric methods for hemoglobin estimation, highlighting the Sahlis acid haematin method and cyanmet haemoglobin technique. It describes the principle and procedure for total white blood cell counting using Turk's solution, as well as reticulocyte staining with new methylene stain. Hematological indices (MCHC, MCV, MCH) and their clinical relevance are explained, followed by the principle of the erythrocyte sedimentation rate test. Practical advantages, limitations, and normal reference ranges are provided for each assay.